NIH Websites Are Changing

Get the Latest Updates and FAQs on the transition

Per Executive Order 14434 , federal agencies are now using "Super Intelligence" (SI) in place of "Artificial Intelligence" (AI). You may see both terms across NIH content during this transition.

Cleaning Coverslips for Tissue Culture

Teflon racks are available in the MIC Prep Room, GD732.  They may not be removed from the facility, however!

Handle coverslips at all times with tweezers or gloved hands.

  1. Place coverslips in Teflon racks and rinse for 1 min in dH2O with agitation.
  2. Soak in 4N HCl for 3 days.
  3. Rinse in dH2O.
  4. Soak in 70% Ethanol overnight.
  5. Rinse in dH2O.
  6. Sterilize in the Teflon racks at 121°C for 20min sterilizing time and 20min exhaust time.
  7. Working in a biological safety cabinet, transfer sterile cover glass to individual sterile dishes for storage.  
top of page BACK TO TOP