Since 2011, the NICHD Microscopy and Imaging Core is organizing annual workshops on microscopy. The two-week workshops are aimed at scientists who want to gain proficiency with light and electron microscopy imaging, sample preparation and image processing. Seminars cover basic and advanced concepts in light and electron microscopy, sample preparation and image analysis. The presentations are delivered by the MIC staff and invited speakers. Lab sessions combine wet labs on different sample preparation techniques, a computer lab on ImageJ, and hands-on on confocal and transmission electron microscopy. The next workshop is scheduled for Spring 2023.

2022 Workshop

Seminars

Monday May 9: 
Light Microscopy 1 - Basic Concepts and Techniques (Vincent Schram, MIC) (PDF 4.4 MB) - B35/GG607, 11 am – 12:30 pm.
Principles of Immunostaining (Ling Yi, MIC) - B35/GG607, 1:30 pm - 3:00 pm.
Electron microscopy - Basic Principles (Chip Dye, MIC) - B35/GG607, 3:00 pm – 4:30 pm.

Tuesday May 10:
Light Microscopy 2 – Confocal, 2P and Light Sheet (Vincent Schram, MIC) (PDF 3.7 MB) - B35/GG607, 11 am – 12:30 pm.
Practical Considerations for Live-Cell Imaging (Josh Pemberton, Balla lab, NICHD) - B35/GG607, 1:30 - 3:00 pm
Electron Microscopy in Life Science - Volume, Correlative and Automated Imaging (Philipp Bastian, Carl Zeiss) - B35/GG607, 3:00 pm- 4:30 pm.

Wednesday May 11:
Light Microscopy 3 - Super-Resolution (Vincent Schram, MIC) (PDF 4.9 MB) – B35/GG607, 11 am – 12:30 pm.
Spatial Mapping of Gene Expression at Single Cell Resolution with RNAscope (Connie Zhang, ACD Bio) -  B35/GG607, 1:30 pm - 3 pm
Practical applications of Artificial Intelligence in light microscopy (Richard Gruskin, Nikon) - B35/GG607, 3:00 pm - 4:30 pm.

Lab Sessions

Thursday May 12: 
ImageJ Computer Lab (Vincent Schram, MIC) (PDF 3.2 MB). Bring your own laptop and learn how to use ImageJ. B35/GG607.
3 hours, 2x20 registrants accepted

Tuesday - Wednesday May 17-18: 
Electron Microscopy Hands-on (Chip Dye, MIC). Learn basic concepts in front of a transmission electron microscope. B35/GD920.
2 hours, 4x3 registrants accepted

Monday – Tuesday May 16-17: 
Immuno-staining of mouse brain cryosections (Ling Yi, MIC). Practice how to stain and mount brain sections for confocal imaging. B35/GD732.
2 x 3 hours over two consecutive days, 2x6 registrants accepted

Wednesday – Thursday May 18-19:
RNAscope staining of mouse brain cryosections (Jade Gibbons, ACD Bio). From bench to microscope, learn and practice how to reveal expression patterns in tissues. B35/GD732.
2 x 3 hours over two consecutive days, 2x6 registrants accepted

Friday May 20: 
Confocal Microscopy Hands-on (Nate Miller, Carl Zeiss, and Andy Ziman, Nikon). Hands-on tutorial on a Zeiss point scanning and a Nikon spinning disk confocal microscope. B35/GD922.
1.5 hours, 4x6 registrants accepted