The Zeiss LSM 800 is a simple and robust point scanning confocal optimized for tiled imaging of large tissue sections. Imaging more than 3 colors is possible but requires switching optical configuration. It is practical only when recording z-stacks.
Laser lines: 405, 488, 561 and 640 nm
Detectors: 3 GAsP PMTs
Emission filters: 2 continuously variable dichroics, 400-700 nm
Objectives: 2.5x/0.07 Plan Neofluar, 5x/0.16 Plan Apochromat, 10x/0.3 Plan Neofluar, 20x/0.8 Plan Apochromat, 40x/0.75 Plan Neofluar, 63x/1.4 Plan-Apochromat
(additional special-purpose objectives are available on request)
The 800 lacks an autofocus device, a transmitted (DIC) detector and a stage-top incubator. Live imaging experiments are not recommended on this system.
Advanced tile functions in Zen Blue include: multiple custom tiles and support point (focus surface)