Zeiss Axioscan 7

Zeiss Axioscan 7 microscope.

The Zeiss Axioscan 7 is a fully automated slide scanner for visible and fluorescence-labelled samples. This scanner is slower than the Axioscan 7, but has a 10-position filter wheel that allows better dye separation. It accepts up to 100 regular 1x3” slides, up to 50 large 2x3” slides, or 25 3x4” slides. Once properly configured, the system automatically loads slides, finds the tissue edges, focus and acquires large tiled images in transmission or wide-field fluorescence. Because the steps normally performed by a human (focusing, finding the tissue edges) are automated, the Axioscan 7 is difficult to configure and troubleshoot. For this reason, end users are instructed to leave their slides with the MIC staff who will scan them at no cost and no additional commitment. Contact Dr. Schram for details.

Objectives: 5x/0.16 Plan Apochromat, 10x/0.5 Plan Apochromat, 20x/0.8 Plan Apochromat, 40x/0.9 Plan Apochromat.

Light source: solid-state Colibri 7 (385, 430, 475, 555, 590, 630 and 735 nm).

Fluorescence channels: Blue, Cyan, Green, Red, m-cherry, Far-red and Cy7.

Single bandpass filter cubes: Blue, Green, Red, Far-red and Cy7.

Multi-bandpass filter cubes: Dapi/Green/Red/Far-red, Dapi/Green/m-Cherry/Cy7 and Cfp/Yfp/m-Cherry

Color camera: Axiocam 705 color, 2464 x 2056 pixels, 3.45um pixel size.

Fluorescence camera: Hamamatsu Orca Flash 4, 2048x2048 pixels, 6.5um pixel size.

Zen Blue with background correction, advanced tiling and z stacks.

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