The Nikon is a unique microscope combining spinning disk confocal imaging with Total Internal reflection Fluorescence (TIRF). TIRF illuminates a 100nm layer at the glass / water interface and is the technique of choice to detect membrane-bound events such as docking, diffusion, endo- and exo-cytosis.
The system is equipped with high-power lasers suitable for localization microscopy such as PALM (endogenous markers) and STORM (chemical dyes) that provide resolutions down to 20 nm.
Outfitted with an autofocus device and a full stage-top incubator, the Nikon spinning disk is suitable for long-term live experiments that can include multi-locations and tiles.
Laser lines: 405, 488, 561 and 640 nm
Confocal scanner: Yokogawa CSU-X1 10,000 rpm
Objectives: 10x/0.45 CFI PlanApo, , 20x/0.75 CFI PlanApo VC, 40x/1.3 CFI PlanFluor (o), 60x/1.4 CFI PlanApo Lambda (o), 100x/1.49 CFI Apo TIRF(o)
(the100x TIRF lens is not stored on the nosepiece, contact staff in advance of your TIRF experiment)
Spinning disk emission filters: 430-480 nm, 450-500 nm, 505-543 nm, 530-580 nm, 570-640 nm, 660-740 nm, dual Fitc/Rhod, dual GFP/mCherry
TIRF emission filters: 450-500 nm, 500-550 nm, 530-580 nm, 575-625 nm, 670-740 nm, dual Fict/Rhod, dual GFP/mCherry
Cameras: dual Andor iXon 897, back-illuminated, 512x512 pixels EM CCDs