Transgenic animals (Tg)

Transgenic animals (Tg) generated with a double promoter construct ( g -crystalline promoter-GFP and heat inducible promoter-GFP) had green eyes and upon heat shock, expressed GFP throughout the animals. In contrast, non-transgenic animals (nTg), which had no GFP in the eyes, had no GFP expression anywhere either before or after heat shock.