Interactive Borrelia burgdorferi B31 Genome Browsers
TSS identification and RNA 5' end mapping:
5' RNA-seq was performed using total RNA isolated from two biological replicates of logarithmic grown spirochetes, in BSKII culture media, at 35°C. Transcription start sites (TSSs) are indicated for each DNA strand and were determined based on a 2-fold enrichment of a 5′ end in the TAP+ tracks (RNA samples incubated with pyrophosphatase) compared to the TAP- tracks. Browser assembled by C. Esnault.
Adams PP, Flores Avile C, Popitsch N, Bilusic I, Schroeder R, Lybecker M, Jewett MW. 2017. In Vivo Expression Technology and 5' End Mapping of the Borrelia burgdorferi Transcriptome Identify Novel RNAs Expressed During Mammalian Infection. Nucleic Acids Research 45(2):775‐792. doi:10.1093/nar/gkw1180