Office of the DiPHR Director (OD) Research: Reproductive Endocrinology

Endometriosis: Natural History, Diagnosis, and Outcomes (ENDO) Study

ENDO Study logoEndometriosis is a gynecologic disorder affecting menstruating women and manifests as ectopic implantation of endometrial glands and stroma outside the uterine cavity. These endometrial implants may respond like normal endometrium to cyclic hormones and may manifest in pain and bleeding. While the etiology of endometriosis remains unknown, evidence is suggesting that endocrine-disrupting chemicals (EDCs) may be associated with the development of endometriosis. The ENDO Study was designed to address key methodologic challenges and to answer questions about the role of EDCs and newly diagnosed endometriosis. Specifically, we sought to assess whether 99 persistent and non-persistent EDCs were associated with endometriosis, and if the findings were consistent across samples and diagnostic methods. The ENDO Study utilized a matched exposure cohort design with surgery being the exposure. The operative sample comprised 495 women age 18 to 44 years undergoing laparoscopy or laparotomy at one of the 14 participating clinical sites in the Salt Lake City or San Francisco areas between 2007 and 2009. The population sample was designed to recruit women at risk for endometriosis (currently menstruating) and diagnosis (residence within clinical centers' catchment areas) from the general population. The population cohort underwent pelvic magnetic resonance imaging (MRI) for the detection of endometriosis in light of having no indications for surgery. 

Following the baseline interview, all women completed standardized anthropometric assessments, the International Physical Activity Questionnaire and the 7-item BECK Depression Inventory Fast Screen before providing blood and urine samples for toxicologic analysis. Women were followed through surgery or MRI for the ascertainment of incident endometriosis.  The incidence of surgically visualized endometriosis for the operative sample was 41% and 11% for the population sample undergoing MRIs (Buck Louis et al., Fertility Sterility 2011).

While most chemical classes of EDCs were associated with a higher odds of an endometriosis diagnosis, blood cadmium and specific congeners of polybrominated biphenyls ethers (PBDEs) and polychlorinated biphyenyls (PCBs) reflected a lower odds of disease. Only two chemical classes (hexachlorocyclohexane (HCH) and phthalates) reflected significantly elevated adjusted odds ratios (ORs) for women in the operative and population samples. In terms of non-persistent chemicals, mono-(2-ethylhexyl) phthalate (mEHP) also was consistently associated with a higher odds of endometriosis irrespective of sampling framework, meaning it was observed in both the operative and population groups (Buck Louis et al., Fertility and Sterility, 2013). Two PFASs were associated with a higher odds of disease in the operative sample:  perfluorooctanoate (PFOA, AOR=1.89; 95% CI 1.17, 3.06) and perfluorononanonoate (PFNA, AOR=2.20; 95% CI 1.02, 4.75) (Buck Louis et al., Epidemiology, 2012).  We also found that one benzophenone UV-type filter (4OH-BP) was associated with endometriosis (AOR=1.19; 95% CI=1.01-1.41 Kunisue et al. 2012) in the population sample.

Principal Investigator

Zhen Chen, Ph.D.

Selected Publications

  • Mumford, S. L., Weck, J., Kannan, K., & Buck Louis, G. M. (2017). Urinary phytoestrogen concentrations are not associated with incident endometriosis in premenopausal women. The Journal of Nutrition, 147(2), 227–234. PMID: 28031375
  • Backonja, U., Hediger, M. L., Chen, Z., Lauver, D. R., Sun, L., Peterson, C. M., & Buck Louis, G. M. (2017). Beyond body mass index: Using anthropometric measures and body composition indicators to assess odds of an endometriosis diagnosis. Journal of women's health (2002), 26(9), 941–950. PMID: 28537460
  • Schliep, K. C., Chen, Z., Stanford, J. B., Xie, Y., Mumford, S. L., Hammoud, A. O., Boiman Johnstone, E., Dorais, J. K., Varner, M. W., Buck Louis, G. M., & Peterson, C. M. (2017). Endometriosis diagnosis and staging by operating surgeon and expert review using multiple diagnostic tools: an inter-rater agreement study. BJOG: An International Journal of Obstetrics and Gynaecology, 124(2), 220–229. PMID: 26435386
  • Buck Louis, G. M., Backonja, U., Schliep, K. C., Sun, L., Peterson, C. M., & Chen, Z. (2016). Women's reproductive history before the diagnosis of incident endometriosis. Journal of Women's Health (2002), 25(10), 1021–1029. PMID: 27379997
  • Schliep, K. C., Mumford, S. L., Johnstone, E. B., Peterson, C. M., Sharp, H. T., Stanford, J. B., Chen, Z., Backonja, U., Wallace, M. E., & Buck Louis, G. M. (2016). Sexual and physical abuse and gynecologic disorders. Human Reproduction (Oxford, England), 31(8), 1904–1912. PMID: 27334336
  • Backonja, U., Buck Louis, G. M., & Lauver, D. R. (2016). Overall adiposity, adipose tissue distribution, and endometriosis: A systematic review. Nursing Research, 65(2), 151–166. PMID: 26938364
  • Kim, E., Zeng, D., & Zhou, X. H. (2015). Semiparametric transformation models for multiple continuous biomarkers in ROC analysis. Biometrical Journal. Biometrische Zeitschrift, 57(5), 808–833. PMID: 26138227 
  • Trabert, B., Chen, Z., Kannan, K., Peterson, C. M., Pollack, A. Z., Sun, L., & Buck Louis, G. M. (2015). Persistent organic pollutants (POPs) and fibroids: Results from the ENDO study. Journal of exposure science & environmental epidemiology, 25(3), 278–285. PMID: 24802554
  • Schliep, K. C., Mumford, S. L., Peterson, C. M., Chen, Z., Johnstone, E. B., Sharp, H. T., Stanford, J. B., Hammoud, A. O., Sun, L., & Buck Louis, G. M. (2015). Pain typology and incident endometriosis. Human Reproduction (Oxford, England), 30(10), 2427–2438. PMID: 26269529
  • Pollack, A. Z., Buck Louis, G. M., Chen, Z., Sun, L., Trabert, B., Guo, Y., & Kannan, K. (2015). Bisphenol A, benzophenone-type ultraviolet filters, and phthalates in relation to uterine leiomyoma. Environmental Research, 137, 101–107. PMID: 25531814
  • Williams, K. E., Miroshnychenko, O., Johansen, E. B., Niles, R. K., Sundaram, R., Kannan, K., Albertolle, M., Zhou, Y., Prasad, N., Drake, P. M., Giudice, L. C., Hall, S. C., Witkowska, H. E., Buck Louis, G. M., & Fisher, S. J. (2015). Urine, peritoneal fluid and omental fat proteomes of reproductive age women: Endometriosis-related changes and associations with endocrine disrupting chemicals. Journal of Proteomics, 113, 194–205. PMID: 25284053
  • Vitonis, A. F., Vincent, K., Rahmioglu, N., Fassbender, A., Buck Louis, G. M., Hummelshoj, L., Giudice, L. C., Stratton, P., Adamson, G. D., Becker, C. M., Zondervan, K. T., Missmer, S. A., & WERF EPHect Working Group (2014). World Endometriosis Research Foundation Endometriosis Phenome and Biobanking Harmonization Project: II. Clinical and covariate phenotype data collection in endometriosis research. Fertility and sterility, 102(5), 1223–1232. PMID: 25256930
  • Vitonis, A. F., Vincent, K., Rahmioglu, N., Fassbender, A., Buck Louis, G. M., Hummelshoj, L., Giudice, L. C., Stratton, P., Adamson, G. D., Becker, C. M., Zondervan, K. T., Missmer, S. A., & WERF EPHect Working Group (2014). World Endometriosis Research Foundation Endometriosis Phenome and Biobanking Harmonization Project: II. Clinical and covariate phenotype data collection in endometriosis research. Fertility and Sterility, 102(5), 1223–1232. PMID: 25256930
  • Johnstone, E. B., Louis, G. M., Parsons, P. J., Steuerwald, A. J., Palmer, C. D., Chen, Z., Sun, L., Hammoud, A. O., Dorais, J., & Peterson, C. M. (2014). Increased urinary cobalt and whole blood concentrations of cadmium and lead in women with uterine leiomyomata: Findings from the ENDO study. Reproductive Toxicology (Elmsford, N.Y.), 49, 27–32. PMID: 24994689
  • Pollack, A. Z., Louis, G. M., Chen, Z., Peterson, C. M., Sundaram, R., Croughan, M. S., Sun, L., Hediger, M. L., Stanford, J. B., Varner, M. W., Palmer, C. D., Steuerwald, A. J., & Parsons, P. J. (2013). Trace elements and endometriosis: the ENDO study. Reproductive Toxicology (Elmsford, N.Y.), 42, 41–48. PMID: 23892002
  • Buck Louis, G. M., Peterson, C. M., Chen, Z., Croughan, M., Sundaram, R., Stanford, J., Varner, M. W., Kennedy, A., Giudice, L., Fujimoto, V. Y., Sun, L., Wang, L., Guo, Y., & Kannan, K. (2013). Bisphenol A and phthalates and endometriosis: the Endometriosis: Natural History, Diagnosis and Outcomes Study. Fertility and Sterility, 100(1), 162–9.e92. PMID: 23579005
  • Steuerwald, A. J., Parsons, P. J., Arnason, J. G., Chen, Z., Peterson, C. M., & Louis, G. M. (2013). Trace element analysis of human urine collected after administration of Gd-based MRI contrast agents: Characterizing spectral interferences using inorganic mass spectrometry. Journal of Analytical Atomic Spectrometry, 28(6), 821–830. PMID: 27397951
  • Peterson, C. M., Johnstone, E. B., Hammoud, A. O., Stanford, J. B., Varner, M. W., Kennedy, A., Chen, Z., Sun, L., Fujimoto, V. Y., Hediger, M. L., Buck Louis, G. M., & ENDO Study Working Group (2013). Risk factors associated with endometriosis: importance of study population for characterizing disease in the ENDO study. American Journal of Obstetrics and Gynecology, 208(6), 451.e1–451.e4511. PMID: 23454253
  • Wolff, E. F., Sun, L., Hediger, M. L., Sundaram, R., Peterson, C. M., Chen, Z., & Buck Louis, G. M. (2013). In utero exposures and endometriosis: the Endometriosis, Natural History, Disease, Outcome (ENDO) study. Fertility and Sterility, 99(3), 790–795. PMID: 23211710
  • Thoma, M. E., Hediger, M. L., Sundaram, R., Stanford, J. B., Peterson, C. M., Croughan, M. S., Chen, Z., Buck Louis, G. M., & ENDO Study Working Group (2012). Comparing apples and pears: Women's perceptions of their body size and shape. Journal of Women's Health (2002), 21(10), 1074–1081. PMID: 22873752
  • Louis, G. M., Peterson, C. M., Chen, Z., Hediger, M. L., Croughan, M. S., Sundaram, R., Stanford, J. B., Fujimoto, V. Y., Varner, M. W., Giudice, L. C., Kennedy, A., Sun, L., Wu, Q., & Kannan, K. (2012). Perfluorochemicals and endometriosis: The ENDO study. Epidemiology (Cambridge, Mass.), 23(6), 799–805. PMID: 22992575
  • Schliep, K. C., Stanford, J. B., Chen, Z., Zhang, B., Dorais, J. K., Boiman Johnstone, E., Hammoud, A. O., Varner, M. W., Louis, G. M., & Peterson, C. M. (2012). Interrater and intrarater reliability in the diagnosis and staging of endometriosis. Obstetrics and Gynecology, 120(1), 104–112. PMID: 22914398
  • Kunisue, T., Chen, Z., Buck Louis, G. M., Sundaram, R., Hediger, M. L., Sun, L., & Kannan, K. (2012). Urinary concentrations of benzophenone-type UV filters in U.S. women and their association with endometriosis. Environmental Science & Technology, 46(8), 4624–4632. PMID: 22417702
  • Buck Louis, G. M., Chen, Z., Peterson, C. M., Hediger, M. L., Croughan, M. S., Sundaram, R., Stanford, J. B., Varner, M. W., Fujimoto, V. Y., Giudice, L. C., Trumble, A., Parsons, P. J., & Kannan, K. (2012). Persistent lipophilic environmental chemicals and endometriosis: The ENDO Study. Environmental Health Perspectives, 120(6), 811–816. PMID: 22417635
  • Buck Louis, G. M., Hediger, M. L., Peterson, C. M., Croughan, M., Sundaram, R., Stanford, J., Chen, Z., Fujimoto, V. Y., Varner, M. W., Trumble, A., Giudice, L. C., & ENDO Study Working Group (2011). Incidence of endometriosis by study population and diagnostic method: The ENDO study. Fertility and Sterility, 96(2), 360–365. PMID: 21719000
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