Key findings and fields of interest

Proteins and Pathways in Developmental Cell-Cell Fusion

• B. Podbilewicz, E. Leikina, A. Sapir, C. Valansi, M. Suissa, G. Shemer, LV Chernomordik. The C. elegans developmental fusogenEFF-1 mediates homotypic fusion in heterologous cells and in vivo. (2006) Developmental Cell 11, 471-481. PMID: 17011487
• T Gattegno, A Mittal, C Valansi, KC Nguyen, DH Hall, LV Chernomordik, B Podbilewicz. Genetic Control of Fusion Pore Expansion in the Epidermis of Caenorhabditis elegans. (2007) Mol Biol Cell 18, 1153-66. PMID: 17229888
• E. Leikina, K. Melikov, S. Sanyal, S.K. Verma, B. Eun, C. Gebert, K. Pfeifer, VA Lizunov, MM Kozlov, LV Chernomordik. Extracellular annexins and dynamin are important for sequential steps in myoblast fusion. (2013) J Cell Biol 200:109-23. PMID: 23277424
• S.K. Verma, E. Leikina, K. Melikov, L.V. Chernomordik. Late stages of the synchronized macrophage fusion in osteoclast formation depend on dynamin. (2014) Biochem. J. 464: 293-300. PMID: 253362564
• S.K. Verma, E. Leikina, K. Melikov, C. Gebert, V. Kram, M.F. Young, B. Uygur, and L.V. Chernomordik. Cell-surface phosphatidylserine regulates osteoclast precursor fusion. J Biol Chem (2018) 293(1):254-270. PMID: 29101233

Anionic Lipids as Cofactors of Viral Entry

• E. Zaitseva, S.T. Yang, K. Melikov, S. Pourmal, L.V. Chernomordik. Dengue virus ensures its fusion in late endosomes using compartment-specific lipids. (2010) PLoS Pathog. 6:e1001131. PMID: 20949067
• J.M. Costin, E. Zaitseva, K.M. Kahle, C.O. Nicholson, D.K. Rowe, A.S. Graham, L.E. Bazzone, G. Hogancamp, M. Figueroa Sierra, R.H. Fong, S.T. Yang, L. Lin, J.E. Robinson, B.J. Doranz, L.V. Chernomordik, S.F. Michael, J.S. Schieffelin, S. Isern. Mechanistic study of broadly neutralizing human monoclonal antibodies against dengue virus that target the fusion loop. (2013) J Virol. 87:52-66. PMID: 23077306
• E. Zaitseva, E. Zaitsev, K. Melikov, A. Arakelyan, M. Marin, R. Villasmil, L.B. Margolis, G.B. Melikyan, L.V. Chernomordik. Fusion Stage of HIV-1 Entry Depends on Virus-Induced Cell Surface Exposure of Phosphatidylserine. (2017) Cell Host Microbe 22:99-110.e7. PMCID: PMC5558241

Cationic Peptides in Innate Immunity and Drug Delivery

• E. Leikina, H. Delanoe-Ayari, K. Melikov, M.-S. Cho, A. Chen, A. J. Waring, W. Wang, Y. Xie, J. A. Loo, R.I. Lehrer, L.V. Chernomordik. Carbohydrate-binding molecules inhibit viral fusion and entry by crosslinking membrane glycoproteins. (2005) Nature Immunology 6:995-1001. PMID:16155572
• J.P. Richard, K. Melikov, E. Vives, C. Ramos, B. Verbeure, M.J. Gait, L.V. Chernomordik and B. Lebleu. Cell-penetrating peptides: a re-evaluation of the mechanism of cellular uptake. (2003) J Biol Chem 278:585-590. PMID: 12411431
• J. P. Richard, K. Melikov, H. Brooks, P. Prevot, B. Lebleu, and L.V. Chernomordik. Cellular uptake of unconjugated TAT peptide involves clathrin-dependent endocytosis and heparan sulfate receptors. (2005) J Biol Chem 280(15):15300-6. PMID: 15687490
• K. Melikov, A. Hara, K. Yamoah, E. Zaitseva, E. Zaitsev, and L.V. Chernomordik. Efficient entry of cell-penetrating peptide nona-arginine into adherent cells involves a transient increase in intracellular calcium. (2015) Biochem. J. 471:221-30. PMID: 26272944

Lipidic Pore as a Bending Deformation

• I.G. Abidor, V.B. Arakelyan, L.V. Chernomordik, Yu.A. Chizmadzhev, V.F. Pastushenko, M.R. Tarasevich. Electrical breakdown of BLM: Main experimental facts and their qualitative discussion. (1979) Bioelectrochemistry & Bioenergetics, v. 6, 37-52.
• L.V. Chernomordik, M.M. Kozlov, G.B. Melikyan, I.G. Abidor, V.S. Markin, Yu.A. Chizmadzhev. The shape of lipid molecules and monolayer membrane fusion. (1985) Biochim. et Biophys. Acta 812:643-655.
• S.I. Sukharev, V.A. Klenchin, S.M. Serov, L.V. Chernomordik, Yu.A. Chizmadzhev. Electroporation and electrophoretic DNA transfer into cells: the effect of DNA interaction with electropores. (1992) Biophys. J. 63:1320-1327.
• K.Ch. Melikov, V. A. Frolov, A. Shcherbakov, A.V. Samsonov, Y.A. Chizmadzhev, LV Chernomordik. Voltage-induced non-conductive pre-pores and metastable single pores in unmodified planar lipid bilayer. (2001) Biophys. J., 80, 1829-1836.

Conserved Fusion-Through-Hemifusion Pathway of Lipid Remodeling in Fusion

• L.V. Chernomordik, S.S. Vogel, E.A. Leikina, A. Sokoloff, H.O. Onoran, and J. Zimmerberg. Lysolipids reversibly inhibit Ca2+, GTP, and pH dependent fusion. (1993) FEBS Letters 318:71-76. PMID: 8436229
• L.V. Chernomordik, V.A. Frolov, E. Leikina, P. Bronk, and J. Zimmerberg. The pathway of membrane fusion catalyzed by influenza hemagglutinin: restriction of lipids, hemifusion, and lipidic fusion pore formation. (1998) J Cell Biol 140:1369-1382. PMID: 9508770
• L.V. Chernomordik, M.M. Kozlov. Protein-Lipid Interplay in Fusion and Fission of Biological Membranes. (2003) Annu Rev Biochem. 72:175-207. PMID: 14527322
• I. Markovic, E. Leikina, M. Zhukovsky, J. Zimmerberg and LV Chernomordik. Synchronized activation and unfolding of influenza virus hemagglutinins in multimeric fusion machine. (2001) J Cell Biol 155:833-844. PMID: 11724823
• E. Zaitseva, A, Mittal, D. Griffin, L.V. Chernomordik. Class II fusion protein of alphaviruses drives membrane fusion through the same pathway as class I proteins. (2005) J Cell Biol 169:167–177. PMID: 15809312