Functional Genomics of Human Neocortical Development: Innovations for Postmortem Tissue Studies

November 14, 2019 (11 a.m.-Noon)

Sponsor/Co-Sponsor(s)

Child Development and Behavior Branch (CDBB), Division of Extramural Research (DER), NICHD

Location

6710B Rockledge Drive, Room 1417, Bethesda, MD, 20817

Purpose

The uniquely human characteristics of our neocortex means that using postmortem tissue can be the most informative way to assay molecular development of this important brain region. However, shifts in regional cell composition over time—such as from increasing gliogenesis over the lifespan—can mask true developmental signal in DNA methylation or transcription when measured in homogenate tissue. Isolating cell types from homogenate is one way to better detect developmental patterns.

In this latest installment of the Advances in Child Development and Behavior Speaker Series Amanda Price, Ph.D. external link, a science and technology policy fellow at the American Association for the Advancement of Science, discusses BrainFlow, an alternative to traditional antibody-based labeling she created for isolating cell types from postmortem brain based on labeling nuclear RNA for fluorescence-activated nuclear sorting. Isolating specific cell populations (e.g., neurons or glia, and even more cell types in the future) from human neocortical tissue can provide increased clarity into both the “when” but also “where” of genomic activity associated with the development of human-specific traits and diseases.

Contacts

For assistance with sign language interpreters and/or other reasonable accommodations to participate in this event:
Laureen Lee, CDBB, DER, NICHD
Phone: 301-496-5578
Email: laureen.lee@nih.gov

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