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Maraia, Richard J

Formal Title:

Senior Investigator

Phone:

301-402-3567

Email:

maraiar@mail.nih.gov

Address:

6 CENTER DR Room 2A02
Bethesda Md 20892
For FedEx use:
Bethesda Md 20892

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Biosketch:

Dr. Richard Maraia is a Senior Investigator and Head of the Section on Molecular and Cell Biology in the Intramural Research Program of the NICHD. Dr. Maraia directs a basic research program in Molecular Genetics that seeks to understand the influences of genetics, biochemistry and cell biology on the programmed metabolism of small nuclear RNAs and messenger RNAs, and how this contributes to growth and development. Of molecular interest is the structural plasticity of the human La antigen and related proteins in their ability to accommodate specific binding to a variety of RNAs that differ in sequence and structure. A major interest is in the biogenesis and metabolism of transfer RNAs, the genetic adapters that translate the genetic code, and the influences of their dynamics and relative abundances on codon bias-driven genetic programs involved in normal growth and in response to the physiologic states of stress and disease.

Dr. Maraia received his B.S. in Biological Sciences from Columbia University and his M.D. from Cornell University Medical College. He obtained specialty training in Pediatrics at The New York Hospital and in Medical Genetics at the NIH. Dr. Maraia's service currently includes Chairmanship of the NIH Regional RNA Club since 2005, and the International Biennial Conference on RNA Polymerases I & III (2010 & 2012), and he is the first President elect of the La and Related Protein (LARP) Society (2010-2014).

Publications (PubMed):

Cellular La protein shields nonsegmented negative-strand RNA viral leader RNA from RIG-I and enhances virus growth by diverse mechanisms.
Conserved and divergent features of the structure and function of La and La-related proteins (LARPs).
It's a mod mod tRNA world.
Precursor-product discrimination by La protein during tRNA metabolism.
Mouse and human La proteins differ in kinase substrate activity and activation mechanism for tRNA processing.
A La-related protein modulates 7SK snRNP integrity to suppress P-TEFb-dependent transcriptional elongation and tumorigenesis.
Mutation of RNA Pol III subunit rpc2/polr3b Leads to Deficiency of Subunit Rpc11 and disrupts zebrafish digestive development.
Conservation of a masked nuclear export activity of La proteins and its effects on tRNA maturation.
Separate RNA-binding surfaces on the multifunctional La protein mediate distinguishable activities in tRNA maturation.
A role for TFIIIC transcription factor complex in genome organization.
The multifunctional RNA-binding protein La is required for mouse development and for the establishment of embryonic stem cells.
The La protein-RNA complex surfaces.
Human La is found at RNA polymerase III-transcribed genes in vivo.
CK2 is responsible for phosphorylation of human La protein serine-366 and can modulate rpL37 5'-terminal oligopyrimidine mRNA metabolism.
Mutations in the RNA polymerase III subunit Rpc11p that decrease RNA 3' cleavage activity increase 3'-terminal oligo(U) length and La-dependent tRNA processing.
Nonphosphorylated human La antigen interacts with nucleolin at nucleolar sites involved in rRNA biogenesis.
RNA polymerase III from the fission yeast, Schizosaccharomyces pombe.
Differential phosphorylation and subcellular localization of La RNPs associated with precursor tRNAs and translation-related mRNAs.
The fission yeast TFIIB-related factor limits RNA polymerase III to a TATA-dependent pathway of TBP recruitment.
BCR/ABL activates mdm2 mRNA translation via the La antigen.
Methylphosphate cap structure in small RNAs reduces the affinity of RNAs to La protein.
Transcription termination by the eukaryotic RNA polymerase III.
RNA polymerase III mutants in TFIIFa-like C37 that cause terminator readthrough with no decrease in transcription output.
tRNAomics: tRNA gene copy number variation and codon use provide bioinformatic evidence of a new anticodon:codon wobble pair in a eukaryote.
Altered nuclear tRNA metabolism in La-deleted Schizosaccharomyces pombe is accompanied by a nutritional stress response involving Atf1p and Pcr1p that is suppressible by Xpo-t/Los1p.
Plasticity and diversity of tRNA anticodon determinants of substrate recognition by eukaryotic A37 isopentenyltransferases.
3' processing of eukaryotic precursor tRNAs.
Point mutations in the Rpb9-homologous domain of Rpc11 that impair transcription termination by RNA polymerase III.
Comparative whole genome sequencing reveals phenotypic tRNA gene duplication in spontaneous Schizosaccharomyces pombe La mutants.
La-related protein 4 binds poly(A), interacts with the poly(A)-binding protein MLLE domain via a variant PAM2w motif, and can promote mRNA stability.
Aberrant nuclear trafficking of La protein leads to disordered processing of associated precursor tRNAs.
La protein and its associated small nuclear and nucleolar precursor RNAs.
Widespread use of TATA elements in the core promoters for RNA polymerases III, II, and I in fission yeast.
Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human.
La protein and the trafficking of nascent RNA polymerase iii transcripts.
Construction of FLAG and histidine tagging vectors for Schizosaccharomyces pombe.
Recognition of nascent RNA by the human La antigen: conserved and divergent features of structure and function.
Control of transfer RNA maturation by phosphorylation of the human La antigen on serine 366.
Isolation and cloning of four subunits of a fission yeast TFIIIC complex that includes an ortholog of the human regulatory protein TFIIICbeta.
Transcription termination by RNA polymerase III in fission yeast. A genetic and biochemically tractable model system.
Terminator-specific recycling of a B1-Alu transcription complex by RNA polymerase III is mediated by the RNA terminus-binding protein La.
5' processing of tRNA precursors can Be modulated by the human La antigen phosphoprotein.
Heterodimer SRP9/14 is an integral part of the neural BC200 RNP in primate brain.
A carboxy-terminal basic region controls RNA polymerase III transcription factor activity of human La protein.
A highly conserved nucleotide in the Alu domain of SRP RNA mediates translation arrest through high affinity binding to SRP9/14.
Vision National Institutes of Health Home BOND National Institues of Health Home Home Storz Lab: Section on Environmental Gene Regulation Home Machner Lab: Unit on Microbial Pathogenesis Home Division of Intramural Population Health Research Home Bonifacino Lab: Section on Intracellular Protein Trafficking Home Lilly Lab: Section on Gamete Development Home Lippincott-Schwartz Lab: Section on Organelle Biology