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Zebrafish Core

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Research Areas

The core's goal is to help researchers of any expertise perform zebrafish experiments aimed at illuminating basic biology and human disease mechanisms, thereby advancing the NIH and NICHD 's research missions.  A typical research question might investigate the function or regulation of a zebrafish gene and/or its ortholog from humans or another species. We are also happy to offer consultation for any other zebrafish-related projects you may be considering. The core is equipped to assist with the following methodologies:

Gene Expression Studies

  • In situ hybridization to characterize developmental expression patterns of zebrafish genes
  • Zebrafish RNA collection for downstream genomic and molecular applications

Fish embryo Side view of fish embryo 

Gain of Gene Function Studies

  • In vitro RNA synthesis
  • RNA and DNA microinjection
  • Transgenic animal production*

Plasmid vector 

Loss of Gene Function Studies

  • Design and microinjection of antisense morpholino oligonucleotides for transient loss of function
  • Design, synthesis and microinjection of TALE nucleases to generate germ-line genetic mutants*

Tale nucleases activity diagram  

Other Methodologies

  • Cryopreservation of transgenic and mutant zebrafish lines
  • Importation of existing zebrafish lines
  • Characterization of phenotypes by time-lapse photography
Last Reviewed: 01/29/2016

​Contact Information

Name: Dr Benjamin Feldman
Zebrafish Core
Phone: 301-435-5556

Staff Directory
Vision National Institutes of Health Home BOND National Institues of Health Home Home Storz Lab: Section on Environmental Gene Regulation Home Machner Lab: Unit on Microbial Pathogenesis Home Division of Intramural Population Health Research Home Bonifacino Lab: Section on Intracellular Protein Trafficking Home Lilly Lab: Section on Gamete Development Home Lippincott-Schwartz Lab: Section on Organelle Biology