Maraia, Richard J
Formal Title:
Senior Investigator
Email:
maraiar@mail.nih.gov
Address:
31 Center Dr Room 2A25, MSC 2426
Bethesda Md 20892-2426
For FedEx use:
Bethesda Md 20892
Biosketch:
Dr. Richard Maraia is a Senior Investigator and Head of the Section on Molecular and Cell Biology in the Intramural Research Program of the NICHD. Dr. Maraia directs a basic research program in Molecular Genetics that seeks to understand the influences of genetics, biochemistry and cell biology on the programmed metabolism of small nuclear RNAs and messenger RNAs, and how this contributes to growth and development. Of molecular interest is the structural plasticity of the human La antigen and related proteins in their ability to accommodate specific binding to a variety of RNAs that differ in sequence and structure. A major interest is in the biogenesis and metabolism of transfer RNAs, the genetic adapters that translate the genetic code, and the influences of their dynamics and relative abundances on codon bias-driven genetic programs involved in normal growth and in response to the physiologic states of stress and disease.
Dr. Maraia received his B.S. in Biological Sciences from Columbia University and his M.D. from Cornell University Medical College. He obtained specialty training in Pediatrics at The New York Hospital and in Medical Genetics at the NIH. Dr. Maraia's service currently includes Chairmanship of the NIH Regional RNA Club since 2005, and the International Biennial Conference on RNA Polymerases I & III (2010 & 2012), and he is the first President elect of the La and Related Protein (LARP) Society (2010-2014).
Publications (PubMed):
| Title |
Authors |
Publication |
Date |
| Cellular La protein shields nonsegmented negative-strand RNA viral leader RNA from RIG-I and enhances virus growth by diverse mechanisms. | Bitko V,Musiyenko A,Bayfield MA,Maraia RJ,Barik S | J Virol | 2008 Aug |
| Conserved and divergent features of the structure and function of La and La-related proteins (LARPs). | Bayfield MA,Yang R,Maraia RJ | Biochim Biophys Acta | 2010 May-Jun |
| It's a mod mod tRNA world. | Maraia RJ,Blewett NH,Bayfield MA | Nat Chem Biol | 2008 Mar |
| Precursor-product discrimination by La protein during tRNA metabolism. | Bayfield MA,Maraia RJ | Nat Struct Mol Biol | 2009 Apr |
| Mouse and human La proteins differ in kinase substrate activity and activation mechanism for tRNA processing. | Park JM,Intine RV,Maraia RJ | Gene Expr | 2007 |
| A La-related protein modulates 7SK snRNP integrity to suppress P-TEFb-dependent transcriptional elongation and tumorigenesis. | He N,Jahchan NS,Hong E,Li Q,Bayfield MA,Maraia RJ,Luo K,Zhou Q | Mol Cell | 2008 Mar 14 |
| Mutation of RNA Pol III subunit rpc2/polr3b Leads to Deficiency of Subunit Rpc11 and disrupts zebrafish digestive development. | Yee NS,Gong W,Huang Y,Lorent K,Dolan AC,Maraia RJ,Pack M | PLoS Biol | 2007 Nov |
| Conservation of a masked nuclear export activity of La proteins and its effects on tRNA maturation. | Bayfield MA,Kaiser TE,Intine RV,Maraia RJ | Mol Cell Biol | 2007 May |
| Separate RNA-binding surfaces on the multifunctional La protein mediate distinguishable activities in tRNA maturation. | Huang Y,Bayfield MA,Intine RV,Maraia RJ | Nat Struct Mol Biol | 2006 Jul |
| A role for TFIIIC transcription factor complex in genome organization. | Noma K,Cam HP,Maraia RJ,Grewal SI | Cell | 2006 Jun 2 |
| The multifunctional RNA-binding protein La is required for mouse development and for the establishment of embryonic stem cells. | Park JM,Kohn MJ,Bruinsma MW,Vech C,Intine RV,Fuhrmann S,Grinberg A,Mukherjee I,Love PE,Ko MS,DePamphilis ML,Maraia RJ | Mol Cell Biol | 2006 Feb |
| The La protein-RNA complex surfaces. | Maraia RJ,Bayfield MA | Mol Cell | 2006 Jan 20 |
| Human La is found at RNA polymerase III-transcribed genes in vivo. | Fairley JA,Kantidakis T,Kenneth NS,Intine RV,Maraia RJ,White RJ | Proc Natl Acad Sci U S A | 2005 Dec 20 |
| CK2 is responsible for phosphorylation of human La protein serine-366 and can modulate rpL37 5'-terminal oligopyrimidine mRNA metabolism. | Schwartz EI,Intine RV,Maraia RJ | Mol Cell Biol | 2004 Nov |
| Mutations in the RNA polymerase III subunit Rpc11p that decrease RNA 3' cleavage activity increase 3'-terminal oligo(U) length and La-dependent tRNA processing. | Huang Y,Intine RV,Mozlin A,Hasson S,Maraia RJ | Mol Cell Biol | 2005 Jan |
| Nonphosphorylated human La antigen interacts with nucleolin at nucleolar sites involved in rRNA biogenesis. | Intine RV,Dundr M,Vassilev A,Schwartz E,Zhao Y,Zhao Y,Depamphilis ML,Maraia RJ | Mol Cell Biol | 2004 Dec |
| RNA polymerase III from the fission yeast, Schizosaccharomyces pombe. | Huang Y,Hamada M,Maraia RJ | Methods Enzymol | 2003 |
| Differential phosphorylation and subcellular localization of La RNPs associated with precursor tRNAs and translation-related mRNAs. | Intine RV,Tenenbaum SA,Sakulich AL,Keene JD,Maraia RJ | Mol Cell | 2003 Nov |
| The fission yeast TFIIB-related factor limits RNA polymerase III to a TATA-dependent pathway of TBP recruitment. | Huang Y,McGillicuddy E,Weindel M,Dong S,Maraia RJ | Nucleic Acids Res | 2003 Apr 15 |
| BCR/ABL activates mdm2 mRNA translation via the La antigen. | Trotta R,Vignudelli T,Candini O,Intine RV,Pecorari L,Guerzoni C,Santilli G,Byrom MW,Goldoni S,Ford LP,Caligiuri MA,Maraia RJ,Perrotti D,Calabretta B | Cancer Cell | 2003 Feb |
| Methylphosphate cap structure in small RNAs reduces the affinity of RNAs to La protein. | Bhattacharya R,Perumal K,Sinha K,Maraia R,Reddy R | Gene Expr | 2002 |
| Transcription termination by the eukaryotic RNA polymerase III. | Arimbasseri AG,Rijal K,Maraia RJ | Biochim Biophys Acta | 2012 Oct 23 |
| RNA polymerase III mutants in TFIIFa-like C37 that cause terminator readthrough with no decrease in transcription output. | Rijal K,Maraia RJ | Nucleic Acids Res | 2012 Oct 23 |
| tRNAomics: tRNA gene copy number variation and codon use provide bioinformatic evidence of a new anticodon:codon wobble pair in a eukaryote. | Iben JR,Maraia RJ | RNA | 2012 Jul |
| Altered nuclear tRNA metabolism in La-deleted Schizosaccharomyces pombe is accompanied by a nutritional stress response involving Atf1p and Pcr1p that is suppressible by Xpo-t/Los1p. | Cherkasova V,Maury LL,Bacikova D,Pridham K,Bähler J,Maraia RJ | Mol Biol Cell | 2012 Feb |
| Plasticity and diversity of tRNA anticodon determinants of substrate recognition by eukaryotic A37 isopentenyltransferases. | Lamichhane TN,Blewett NH,Maraia RJ | RNA | 2011 Oct |
| 3' processing of eukaryotic precursor tRNAs. | Maraia RJ,Lamichhane TN | Wiley Interdiscip Rev RNA | 2011 May |
| Point mutations in the Rpb9-homologous domain of Rpc11 that impair transcription termination by RNA polymerase III. | Iben JR,Mazeika JK,Hasson S,Rijal K,Arimbasseri AG,Russo AN,Maraia RJ | Nucleic Acids Res | 2011 Aug |
| Comparative whole genome sequencing reveals phenotypic tRNA gene duplication in spontaneous Schizosaccharomyces pombe La mutants. | Iben JR,Epstein JA,Bayfield MA,Bruinsma MW,Hasson S,Bacikova D,Ahmad D,Rockwell D,Kittler EL,Zapp ML,Maraia RJ | Nucleic Acids Res | 2011 Jun |
| La-related protein 4 binds poly(A), interacts with the poly(A)-binding protein MLLE domain via a variant PAM2w motif, and can promote mRNA stability. | Yang R,Gaidamakov SA,Xie J,Lee J,Martino L,Kozlov G,Crawford AK,Russo AN,Conte MR,Gehring K,Maraia RJ | Mol Cell Biol | 2011 Feb |
| Aberrant nuclear trafficking of La protein leads to disordered processing of associated precursor tRNAs. | Intine RV,Dundr M,Misteli T,Maraia RJ | Mol Cell | 2002 May |
| La protein and its associated small nuclear and nucleolar precursor RNAs. | Maraia RJ,Intine RV | Gene Expr | 2002 |
| Widespread use of TATA elements in the core promoters for RNA polymerases III, II, and I in fission yeast. | Hamada M,Huang Y,Lowe TM,Maraia RJ | Mol Cell Biol | 2001 Oct |
| Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human. | Huang Y,Maraia RJ | Nucleic Acids Res | 2001 Jul 1 |
| La protein and the trafficking of nascent RNA polymerase iii transcripts. | Maraia RJ | J Cell Biol | 2001 May 14 |
| Construction of FLAG and histidine tagging vectors for Schizosaccharomyces pombe. | Huang Y,Hamada M,Patel J,Maraia RJ | Yeast | 2001 Mar 30 |
| Recognition of nascent RNA by the human La antigen: conserved and divergent features of structure and function. | Maraia RJ,Intine RV | Mol Cell Biol | 2001 Jan |
| Control of transfer RNA maturation by phosphorylation of the human La antigen on serine 366. | Intine RV,Sakulich AL,Koduru SB,Huang Y,Pierstorff E,Goodier JL,Phan L,Maraia RJ | Mol Cell | 2000 Aug |
| Isolation and cloning of four subunits of a fission yeast TFIIIC complex that includes an ortholog of the human regulatory protein TFIIICbeta. | Huang Y,Hamada M,Maraia RJ | J Biol Chem | 2000 Oct 6 |
| Transcription termination by RNA polymerase III in fission yeast. A genetic and biochemically tractable model system. | Hamada M,Sakulich AL,Koduru SB,Maraia RJ | J Biol Chem | 2000 Sep 15 |
| Terminator-specific recycling of a B1-Alu transcription complex by RNA polymerase III is mediated by the RNA terminus-binding protein La. | Goodier JL,Maraia RJ | J Biol Chem | 1998 Oct 2 |
| 5' processing of tRNA precursors can Be modulated by the human La antigen phosphoprotein. | Fan H,Goodier JL,Chamberlain JR,Engelke DR,Maraia RJ | Mol Cell Biol | 1998 Jun |
| Heterodimer SRP9/14 is an integral part of the neural BC200 RNP in primate brain. | Kremerskothen J,Zopf D,Walter P,Cheng JG,Nettermann M,Niewerth U,Maraia RJ,Brosius J | Neurosci Lett | 1998 Apr 10 |
| A carboxy-terminal basic region controls RNA polymerase III transcription factor activity of human La protein. | Goodier JL,Fan H,Maraia RJ | Mol Cell Biol | 1997 Oct |
| A highly conserved nucleotide in the Alu domain of SRP RNA mediates translation arrest through high affinity binding to SRP9/14. | Chang DY,Newitt JA,Hsu K,Bernstein HD,Maraia RJ | Nucleic Acids Res | 1997 Mar 15 |
|
|
|