Figure 1. T cell antigen receptors expressed in 6Y/6Y and 6F/6F knock-in mice Subunit composition of the T cell antigen receptors in 6Y/6Y and 6F/6F mice. 6Y/6Y mice express wild-type zeta chain dimers with functional ITAM signaling motifs that contain two tyrosine (Y) residues. 6F/6F mice express mutant zeta chain dimers in which the ITAM tyrosines have been changed to phenylalanine (F).
Much of our research focuses on the role of TCR signal transduction in thymocyte development. Signal transduction sequences (termed immunoreceptor tyrosine-based activation motifs or ITAMs) are contained within four distinct subunits of the multimeric TCR complex (zeta, CD3-gamma, CD3-delta, and CD3-epsilon). Di-tyrosine residues within ITAMs are phosphorylated upon TCR engagement; their function is to recruit signaling molecules, such as protein tyrosine kinases, to the TCR complex, thereby initiating the T cell activation cascade. Though conserved, ITAM sequences are nonidentical, raising the possibility that the diverse developmental and functional responses controlled by the TCR may be partly regulated by distinct ITAMs. We previously generated zeta-deficient and CD3-epsilon–deficient mice by gene targeting. We genetically reconstituted the mice with transgenes encoding wild-type or signaling-deficient (ITAM–mutant) forms of zeta and CD3-epsilon and characterized the developmental and functional consequences of these alterations for TCR signaling. We found that TCR-ITAMs are functionally equivalent but act in concert to amplify TCR signals and that TCR signal amplification is critical for thymocyte selection, the process by which potentially useful immature T cells are instructed to survive and differentiate further (positive selection) and by which potentially auto-reactive cells that may cause auto-immune disease are deleted in the thymus (negative selection). Unexpectedly, we found that multiple TCR-ITAMs are not required for mature T cell effector functions. One possible explanation is that ITAM–mediated signal amplification is not required for mature T cell activation; another is that, in ITAM–mutant mice, T cells exhibit normal functional responsiveness because of compensatory mechanisms imposed during selection. To resolve this question, we recently generated a TCR–zeta chain conditional knockin mouse in which T cell development and selection can occur without attenuation of TCR signaling (i.e., in the presence of wild-type 3 ITAM "6Y" zeta chain), but in which mature, post-selection T cells may be induced to express TCRs containing signaling-defective (0 ITAM "6F") zeta chains in lieu of wild-type zeta chain (Figure 1). Thus, mature T cell signaling should not be influenced by potential compensatory mechanisms that operate during T cell maturation such that T cells in these mice should be faithful indicators of the role of multiple TCR ITAMs in mediating specific, mature T cell responses. Preliminary experiments with the mice confirmed that the knockin zeta locus functions as predicted. We are currently using this model system to evaluate the role of ITAM multiplicity and ITAM–mediated signal amplification in T cell development, immune tolerance, and mature T cell function.
Figure 2. Themis is highly conserved in vertebrates. Themis contains two novel CABIT domains, each with a conserved cysteine (red) and conserved flanking residues (yellow), a nuclear localization signal (NLS), and Proline Rich Region (PRR).
Using a subtractive cDNA library–screening approach, we recently identified Themis, a novel T cell–specific protein (Figure 2). To investigate the function of the protein in T cell signaling and development, we generated Themis-knockdown cell lines, Themis-knockout mice, and Themis-transgenic mice. Analysis of the effects of modulating Themis expression revealed a critical role for the protein in late T cell development. Current data indicate that Themis functions in the signaling pathway downstream of the T cell receptor (TCR), perhaps by integrating or sustaining TCR signaling. Ongoing studies focus on elucidating the mechanism through which Themis regulates T cell development.
Figure 3. Model of Ldb1 function in the hematopoietic lineage
Ldb1 forms a multimeric DNA–binding complex in hematopoietic cells with the adapter Lmo2 and the transcription factors Scl and Gata1 or Gata2. In hematopoietic stem cells (HSCs), in which Gata2 is highly expressed, Ldb1-Lmo2-Scl-Gata2 complexes positively regulate expression of HSC maintenance genes. Differentiation of HSCs to the myeloid or lymphoid lineage (LMPP) is trigged by downregulation of Ldb1 whereas commitment to the erythroid lineage (ery) is triggered by induction of Gata1 and downregulation of Gata2, resulting in the formation of an Ldb1-Lmo2-Scl-Gata1 complex that positively regulates expression of erythroid-specific genes.
Lim domain binding protein-1 (Ldb1) is a ubiquitously expressed nuclear protein that contains a LIM–zinc finger protein interaction motif and a dimerization domain. In hematopoietic cells, Ldb1 functions by interacting with and/or recruiting specific partners (including the LIM–only protein LMO2 and the transcription factors SCL and GATA1 or GATA2) to form multimolecular transcription complexes (Figure 3). Within the hematopoietic lineage, expression of Ldb1 is highest in progenitors, which include hematopoietic stem cells (HSCs). Ldb1−/− mice die between day 9 and 10 of gestation, preventing us from directly studying the impact of loss of Ldb1 on fetal or adult hematopoiesis. We investigated the role of Ldb1 in hematopoiesis by following the fate of Ldb1–null embryonic stem (ES) cells in mouse blastocyst chimeras and by conditional, stage-specific deletion of Ldb1. Significantly, Ldb1–null ES cells were capable of generating HSCs, which could give rise to both myeloid and lymphoid lineage cells; however, the number of Ldb1−/− HSCs gradually diminished at later stages of development. Following adoptive transfer of fetal liver cells, Ldb1–null HSCs were rapidly lost over a period of three months, indicating a failure of self-renewal or survival. More recent data indicate that the loss of Ldb1–null HSCs results from differentiation rather than cell death. Although expressed in ES cells, Ldb1 expression is not required for ES maintenance, indicating a selective requirement in adult stem cell populations. We performed a genome-wide screen for Ldb1–binding sites using ChIP-seq high-throughput sequencing. Analysis of ChIP-seq data revealed that Ldb1 binds at the promoter or regulatory sequences near a large number of genes known to be required for HSC maintenance. The data suggest that Ldb1 complexes may function in a manner similar to Oct4/nanog/Sox2 in ES cells to regulate a core transcriptional network required for stem cell maintenance. Examination of the function of Ldb1 in lineages downstream of the HSC identified an essential function in the erythroid lineage but not in other myeloid cells or lymphoid cells. Interestingly, ChIP-seq analysis of Ldb1 DNA–binding complexes in erythroid cells demonstrated that Ldb1 complexes contain Gata1 (which is highly expressed in the erythroid lineage) instead of Gata2. The results suggest that multimeric Ldb1 transcription complexes may have distinct functions in the hematopoietic system depending upon their subunit composition, with Gata2–containing complexes regulating expression of HSC maintenance genes and Gata1 complexes regulating expression of erythroid-specific genes (Figure 3).
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