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DePamphilis Lab: Section on Eukaryotic DNA Replication

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Research Techniques

Techniques Used In Our Research

  • cloning genes in bacteria, yeast, insect viruses, and mammalian cells
  • DNA purification
  • DNA sequence analysis
  • DNA methylation analysis
  • DNA combing analysis
  • quantifiable PCR applications
  • expression of genes in animal cells
  • blotting-hybridization of DNA with RNA ("Northern" blot) or DNA ("Southern blot")
  • microinjection of DNA and proteins into mouse embryos
  • construction of transgenic and knock-out mice
  • confocal fluorescence microscopy
  • cell fractionation
  • protein purification
  • blotting-antibody staining of proteins ("Western blot")
  • affinity labeling of proteins
  • mapping protein - protein and protein - DNA interactive domains
  • protein foot-printing in vitro and in vivo
  • chromatin immuno-precipitation assays
  • growth and characterization of mammalian cells in culture
  • growth and characterization of animal viruses in cell cultures
  • preparation of cell extracts that can replicate DNA in vitro
  • identification and characterization of nascent DNA in vitro and in vivo
Last Updated Date: 11/30/2012
Last Reviewed Date: 11/30/2012

Contact Information

Name: Prof Melvin L Depamphilis
Senior Investigator
Section on Eukaryotic DNA Replication
Phone: 301-402-8234
Fax: 301-480-9354
Email: depamphm@mail.nih.gov

Staff Directory

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