DePamphilis Lab: Section on Eukaryotic DNA Replication
Page Content
Research Techniques
Techniques Used In Our Research
- cloning genes in bacteria, yeast, insect viruses, and mammalian cells
- DNA purification
- DNA sequence analysis
- DNA methylation analysis
- DNA combing analysis
- quantifiable PCR applications
- expression of genes in animal cells
- blotting-hybridization of DNA with RNA ("Northern" blot) or DNA ("Southern blot")
- microinjection of DNA and proteins into mouse embryos
- construction of transgenic and knock-out mice
- confocal fluorescence microscopy
- cell fractionation
- protein purification
- blotting-antibody staining of proteins ("Western blot")
- affinity labeling of proteins
- mapping protein - protein and protein - DNA interactive domains
- protein foot-printing in vitro and in vivo
- chromatin immuno-precipitation assays
- growth and characterization of mammalian cells in culture
- growth and characterization of animal viruses in cell cultures
- preparation of cell extracts that can replicate DNA in vitro
- identification and characterization of nascent DNA in vitro and in vivo
Last Updated Date: 11/30/2012