Because of limited staff, the core favors a team-based approach for more involved projects. A team-based approach means that the core will provide training, access to equipment and standard reagents and quality-control feedback to someone from the recipient laboratory who will independently perform key parts of the needed experiments. This policy is essential to ensure that the core can serve everybody's needs. Shorter projects requiring just a few rounds of a single procedure, or work with regulated animals in the case of non-licensed researchers, will be performed exclusively by the core's staff.
Good core citizenship means reserving and logging the time that you use core equipment, proper use of the equipment and being mindful of others in the core's shared space. To gain access, please return a signed copy of the NICHD Zebrafish Core User Agreement to Ben Feldman.
Core costs and pricing are still being evaluated. At present, consultation and training time, common reagents and instrument use are all free of charge but laboratories will be asked to pay for costly consumables.
To help ensure our continued funding, it is critical that recognition be given on publications that include data generated with the core's assistance. Whether this recognition comes as co-authorship for a core staff member or as an acknowledgement should depend upon the significance of the core's contribution.
Please contact the core with new link suggestions or questions about how to use these resources.
ZFIN , The University of Oregon's Zebrafish Information Network
Zebrafish Interest Group
Ensembl's Zebrafish Genome Browser UCSC's Zebrafish Genome Browser
ZFIN's General Fish Database
The University of Oregon's Zebrafish International Resource Center Homepage & General Fish Database
The Sanger Institute's Zebrafish Mutation Project Homepage & Fish Knockout Database
The Fred Hutchinson Cancer Research Center Moens TILLing Project Homepage & Fish Knockout Database The Institute of Molecular and Cell Biology's Zebrafish Enhancer TRAP lines database Homepage & Transgenic Fish Database Center for Regenerative Therapies Dresden's CreZoo Transgenic Fish Database
Below is an overview of the steps we undertake for TALEN-based mutagenesis in the NICHD zebrafish core. Several specific websites are mentioned in these pages. There are certainly alternate strategies, websites and web-based tools that can achieve the same goals. In no way do we wish to advocate one of these over another. These are simply the approaches that the NICHD Zebrafish Core currently employs. Researchers interested in minimizing efforts should be aware that several companies offer to design and synthesize TALENs for a fee.
Finding zebrafish orthologs to genes from other speciesDeciding where to make the mutationDetecting mutationsTALEN Bibliography
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