Yijing Chen, Jay Vivian, Della Yee, Elizabeth Schneider and Terry MagnusonDepartment of Genetics, University of North Carolina, Chapel Hill, NC
To take advantage of the mutagenicity of ENU and its ability to create allelic series of mutations, we have developed an approach for generating these mutations using mouse embryonic stem (ES) cells. A high mutation frequency can be achieved, and modulating DNA repair activities can enhance this frequency. The treated cells retain germ line competency, thereby rendering this approach applicable for efficient, high-throughput generation of allelic series of mutations pivotal for a fine-tuned dissection of biological pathways. Mutagenesis in ES cells represents an important addition to the existing strategies to deliver an expanded repertoire of mouse mutants.
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